AlleleSeq
From GersteinInfo
(→General outline of pipeline) |
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(2) '''AlleleSeq pipeline - mapping and statistical testing using PIPELINE.mk package''' <br> | (2) '''AlleleSeq pipeline - mapping and statistical testing using PIPELINE.mk package''' <br> | ||
| - | + | (a) Reads from ChIP-seq and RNA-seq experiments are aligned and mapped to both | |
haplotype genomes. <br><br> | haplotype genomes. <br><br> | ||
| - | + | (b) Then for each SNV position with mapped reads, we compare the allele | |
frequencies observed in the two parental haplotypes. <br><br> | frequencies observed in the two parental haplotypes. <br><br> | ||
Revision as of 15:53, 7 June 2013
Contents |
General outline of pipeline
The basic goal of the pipeline is to take a large collection of reads generated from ChIP-seq or RNA-seq experiments associated with an individual and detect single nucleotide variants (SNVs) that correspond to significantly skewed number of reads. To do this, the pipeline starts with a preprocessing step, before the actual process.
(1) Pre-processing - diploid genome construction using vcf2diploid
In the Rozowsky et al. (2011) paper, the
pre-processing step separate (phase) the child's diploid genome into its parental
haplotypes based on the sequences of the parents.
(2) AlleleSeq pipeline - mapping and statistical testing using PIPELINE.mk package
(a) Reads from ChIP-seq and RNA-seq experiments are aligned and mapped to both
haplotype genomes.
(b) Then for each SNV position with mapped reads, we compare the allele
frequencies observed in the two parental haplotypes.
