VAT/download

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(Setup of the web server)
 
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* [http://hgwdev.cse.ucsc.edu/~kent/exe/linux/blatSuite.34.zip BlatSuite] - BLAT and a collection of utility programs. These tools are utilized by VAT. Note: these executables must be part of the PATH.  
* [http://hgwdev.cse.ucsc.edu/~kent/exe/linux/blatSuite.34.zip BlatSuite] - BLAT and a collection of utility programs. These tools are utilized by VAT. Note: these executables must be part of the PATH.  
* [http://www.libgd.org/Main_Page GD library] - The GD library is used to create an image for each gene model and its associated variants (version-2.0.35; required by VAT).
* [http://www.libgd.org/Main_Page GD library] - The GD library is used to create an image for each gene model and its associated variants (version-2.0.35; required by VAT).
-
* [http://samtools.sourceforge.net/tabix.shtml Tabix] - Tabix is generic tool that indexes position-sorted files in tab-delimited formats to facilitate fast retrieval ([http://sourceforge.net/projects/samtools/files/tabix/ download]). These tools are utilized by VAT. Note: these executables must be part of the PATH.
+
* [http://samtools.sourceforge.net/tabix.shtml Tabix] - Tabix (version-0.2.3) is a generic tool that indexes position-sorted files in tab-delimited formats to facilitate fast retrieval ([http://sourceforge.net/projects/samtools/files/tabix/ download]). These tools are utilized by VAT. Note: these executables must be part of the PATH.
-
 
+
* [http://rna.urmc.rochester.edu/RNAstructure.html RNAstructure] - RNAstructure is a software package for RNA structure prediction and analysis. This tool is utilized by VAT for prediction of structures for RNA sequences with and without the variants.
 +
* [http://varna.lri.fr/downloads.html VARNA] - VARNA is a java applet for producing high quality RNA secondary structure plots. VAT utilizes VARNA for visualization of the RNA secondary structures.
<br>
<br>
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VAT is a based on a general C library, called libBIOS.  A TAR ball of libBIOS and VAT can be downloaded here:
VAT is a based on a general C library, called libBIOS.  A TAR ball of libBIOS and VAT can be downloaded here:
-
* [http://archive.gersteinlab.org/proj/VAT/src libBIOS-1.1.0.tar.gz]  
+
* [http://homes.gersteinlab.org/people/lh372/VAT/libbios-1.0.0.tar.gz libbios-1.0.0.tar.gz] - Initial upload (6/14/2011)
-
* [http://archive.gersteinlab.org/proj/VAT/src VAT-0.5.tar.gz] - Initial upload
+
* [http://homes.gersteinlab.org/people/lh372/VAT/vat-1.0.0.tar.gz vat-1.0.0.tar.gz] - Initial upload (6/14/2011)
<br>
<br>
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Statically built binaries for UNIX can be found here:
Statically built binaries for UNIX can be found here:
-
* [http://archive.gersteinlab.org/proj/VAT/src VAT-0.5-UNIX.tar.gz] - 64bit version
+
* [http://homes.gersteinlab.org/people/lh372/VAT/vat-1.0.0_64bit.zip vat-1.0.0_64bit.zip] - Initial upload (6/14/2011)
<br>
<br>
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=== Installation of the external GSL and GD libraries ===
=== Installation of the external GSL and GD libraries ===
-
In order to install VAT two external libraries must be installed first. The libBIOS library depends on GSL, wheres VAT makes use of the GD library. Please follow the instructions provided by each package. The GSL library can be installed on most systems using the following commands (for details, please refer to the specific instructions at the [http://www.gnu.org/software/gsl/ GNU Scientific Library] website):
+
In order to install VAT two external libraries must be installed first. The libBIOS library depends on GSL, whereas VAT makes use of the GD library. Please follow the instructions provided by each package. The GSL library can be installed on most systems using the following commands (for details, please refer to the specific instructions at the [http://www.gnu.org/software/gsl/ GNU Scientific Library] website):
<pre>
<pre>
-
$ cd /path/to/gsl/
+
$ cd /path/to/gsl-1.14/
$ ./configure --prefix=`pwd`
$ ./configure --prefix=`pwd`
$ make
$ make
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Similarly, the [http://www.libgd.org/Main_Page GD library] can be installed on most systems with the following commands:
Similarly, the [http://www.libgd.org/Main_Page GD library] can be installed on most systems with the following commands:
<pre>
<pre>
-
$ cd /path/to/gd/
+
$ cd /path/to/gd-2.0.35/
$ ./configure --prefix=`pwd` --with-jpeg=/path/to/jpegLib/
$ ./configure --prefix=`pwd` --with-jpeg=/path/to/jpegLib/
$ make
$ make
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libBIOS can be installed on most systems with the following commands:
libBIOS can be installed on most systems with the following commands:
<pre>
<pre>
-
$ cd /path/to/libbios/
+
$ cd /path/to/libbios-x.x.x/
$ ./configure --prefix=`pwd`  
$ ./configure --prefix=`pwd`  
$ make
$ make
$ make install
$ make install
</pre>
</pre>
 +
 +
<br>
 +
 +
<center>[[#top|Top]]</center>
 +
 +
=== Installation of RNAstructure and VARNA ===
 +
 +
Download RNAstructure and follow the building instructions. Make sure that the build directory is included in PATH environment variable. In addition, RNAstructure needs an environment variable named DATAPATH to be set to the directory of thermodynamic parameter files that are distributed with RNAstructure package.
 +
 +
Download VARNA jar file and add the jar file path to CLASSPATH environment variable.
<br>
<br>
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A few simple steps are required to install VAT:
A few simple steps are required to install VAT:
<pre>
<pre>
-
$ cd /path/to/vat/
+
$ cd /path/to/vat-x.x.x/
$ ./configure --prefix=`pwd`  
$ ./configure --prefix=`pwd`  
$ make
$ make
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</pre>
</pre>
-
VAT contains a configuration file (vatConfirguration.txt), which contains a set of variables that are used by a number of different programs. The name/value pairs are space or tab-delimited. Empty lines are lines starting with '//' are ignored.
+
VAT contains a configuration file ('''vatConfirgurationTemplate.txt'''), which contains a set of variables that are used by a number of different programs. The name/value pairs are space or tab-delimited. Empty lines are lines starting with '//' are ignored.
<pre>
<pre>
-
// Tabix directory
+
// ===============================================================================
-
// ===============
+
// REQUIRED
 +
// ===============================================================================
-
TABIX_DIR /net/gerstein/lh372/SOFT/tabix-0.2.3
+
// Tabix directory (includes both tabix and bgzip)
 +
TABIX_DIR /path/to/tabix-0.2.3
 +
// ===============================================================================
 +
// OPTIONAL (required only for CGIs)
 +
// ===============================================================================
-
// CGI base URL
+
// CGI base URL (where the CGIs are located)
-
// ============
+
WEB_URL_CGI http://webserver.org/path
-
WEB_URL_CGI http://dynamic.gersteinlab.org/people/lh372/dev
+
// Path to the web data directory where the preprocessed files are stored
 +
WEB_DATA_DIR /path/to/public_html/path/to/VAT
 +
// URL to preprocessed files
 +
WEB_DATA_URL http://webserver.org/path/to/VAT
 +
</pre>
 +
This file has to be '''configured properly''' by filling in the required information. Subsequently, the following environment variable ('''VAT_CONFIG_FILE''') has to be set:
-
// Path to the web data directory where the preprocessed files are stored
+
VAT_CONFIG_FILE=/pathTo/vat/vatConfirgurationTemplate.txt
-
// ======================================================================
+
-
WEB_DATA_DIR /home/lh372/public_html/VAT
+
<br><br>
 +
== Setup of the web server ==
 +
<br>
-
// URL to preprocessed files
+
This step is optional, but useful for visualizing the results of processed data sets. The following steps are required:
-
// =========================
+
-
WEB_DATA_URL http://homes.gersteinlab.org/people/lh372/VAT
+
* The executable '''vat_cgi''' has to be located in the cgi-bin directory on the web server
 +
* The configuration file ('''vatConfirgurationTemplate.txt''') must contain the pertinent information
 +
* The following .htaccess file should be added to the cgi-bin:
 +
SetEnv VAT_CONFIG_FILE /path/to/vatConfirgurationTemplate.txt
 +
* The web data directory (defined by WEB_DATA_DIR in the configuration file) requires the following information:
 +
** '''Pre-processed annotation sets'''
 +
** The '''tabix''' and '''bgzip''' executables
 +
** Two images provided by the VAT source code: '''check.png''' and '''processing.gif''' (referred to by vat_cgi)
 +
** Directory that has the same name as the data set (in this example: SampleData). This directory contains the images for each gene (created by [http://info.gersteinlab.org/VAT#vcf2images vcf2images]) and a VCF file for each gene (created by [http://info.gersteinlab.org/VAT#vcfSubsetByGene vcfSubsetByGene])
 +
*** SampleData
 +
*** SampleData.vcf.gz
 +
*** SampleData.vcf.gz.tbi
 +
*** SampleData.sampleSummary.txt (generated by [http://info.gersteinlab.org/VAT#vcfSummary vcfSummary])
 +
*** SampleData.geneSummary.txt (generated by [http://info.gersteinlab.org/VAT#vcfSummary vcfSummary])
 +
** The construction of the vat_cgi URL requires following item/value pairs (for example: http://dynamic.gersteinlab.org/people/lh372/vat_cgi?mode=process&dataSet=ALL.2of4intersection.20100804.chr22&annotationSet=gencode3c&type=coding):
 +
*** mode=process
 +
*** dataSet=SampleData
 +
*** annotationSet=nameOfAnnotationSet
 +
*** type=coding or nonCoding
-
</pre>
+
For additional information please refer to the [http://info.gersteinlab.org/VAT#Setting_up_the_web_server example workflow].
-
 
+
-
The [http://info.gersteinlab.org/VAT#Setting_up_the_web_server example workflow] shows how to set up the web server.
+
<br><br>
<br><br>
-
== Download of preprocessed annotation sets ==
+
== Download of pre-processed annotation sets ==
 +
<br>
-
The following annotation sets are derived from the [http://www.gencodegenes.org/ GENCODE]:
+
The following annotation sets are derived from the [http://www.gencodegenes.org/ GENCODE] project. Each each entry has a set of '''transcript coordinates''' (in [http://info.gersteinlab.org/VAT#Interval Interval] format) and a set of '''transcript sequences''' (introns removed; sequence with respect to the '+' strand; in FASTA format)
 +
 
 +
* Coding sequence (CDS) elements where the both the ''gene_type'' and ''transcript_type'' are ''protein_coding'':
 +
** GENCODE version 3b (hg18): [http://homes.gersteinlab.org/people/lh372/VAT/gencode3b.interval Transcript coordinates], [http://homes.gersteinlab.org/people/lh372/VAT/gencode3b.fa Transcript sequences]
 +
** GENCODE version 3c (hg19): [http://homes.gersteinlab.org/people/lh372/VAT/gencode3c.interval Transcript coordinates], [http://homes.gersteinlab.org/people/lh372/VAT/gencode3c.fa Transcript sequences]
 +
** GENCODE version 4 (hg19): [http://homes.gersteinlab.org/people/lh372/VAT/gencode4.interval Transcript coordinates], [http://homes.gersteinlab.org/people/lh372/VAT/gencode4.fa Transcript sequences]
 +
** GENCODE version 5 (hg19): [http://homes.gersteinlab.org/people/lh372/VAT/gencode5.interval Transcript coordinates], [http://homes.gersteinlab.org/people/lh372/VAT/gencode5.fa Transcript sequences]
 +
** GENCODE version 6 (hg19): [http://homes.gersteinlab.org/people/lh372/VAT/gencode6.interval Transcript coordinates], [http://homes.gersteinlab.org/people/lh372/VAT/gencode6.fa Transcript sequences]
 +
** GENCODE version 7 (hg19): [http://homes.gersteinlab.org/people/lh372/VAT/gencode7.interval Transcript coordinates], [http://homes.gersteinlab.org/people/lh372/VAT/gencode7.fa Transcript sequences]
 +
 
 +
<br>
-
* GENCODE version 3b (hg18) - includes coding sequence (CDS) elements where the both the gene_type and transcript_type are protein_coding
+
* miRNAs where ''gene_type'' is ''miRNA'':
-
** [http://homes.gersteinlab.org/people/lh372/VAT/gencode3b.interval gencode3b.interval] - [http://info.gersteinlab.org/VAT#Interval Interval] file with transcript coordinates
+
** GENCODE version 3b (hg18): [http://homes.gersteinlab.org/people/lh372/VAT/gencode3b.miRNA.interval Transcript coordinates], [http://homes.gersteinlab.org/people/lh372/VAT/gencode3b.miRNA.fa Transcript sequences]
-
** [http://homes.gersteinlab.org/people/lh372/VAT/gencode3b.fa gencode3b.fa] - FASTA file with transcript sequences (introns are removed; sequence with respect to the '+' strand)
+
** GENCODE version 3c (hg19): [http://homes.gersteinlab.org/people/lh372/VAT/gencode3c.miRNA.interval Transcript coordinates], [http://homes.gersteinlab.org/people/lh372/VAT/gencode3c.miRNA.fa Transcript sequences]
-
* GENCODE version 3c (hg19) - includes coding sequence (CDS) elements where the both the gene_type and transcript_type are protein_coding
+
** GENCODE version 4 (hg19): [http://homes.gersteinlab.org/people/lh372/VAT/gencode4.miRNA.interval Transcript coordinates], [http://homes.gersteinlab.org/people/lh372/VAT/gencode4.miRNA.fa Transcript sequences]
-
** [http://homes.gersteinlab.org/people/lh372/VAT/gencode3c.interval gencode3c.interval] - [http://info.gersteinlab.org/VAT#Interval Interval] file with transcript coordinates
+
** GENCODE version 5 (hg19): [http://homes.gersteinlab.org/people/lh372/VAT/gencode5.miRNA.interval Transcript coordinates], [http://homes.gersteinlab.org/people/lh372/VAT/gencode5.miRNA.fa Transcript sequences]
-
** [http://homes.gersteinlab.org/people/lh372/VAT/gencode3c.fa gencode3c.fa] - FASTA file with transcript sequences (introns are removed; sequence with respect to the '+' strand)
+
** GENCODE version 6 (hg19): [http://homes.gersteinlab.org/people/lh372/VAT/gencode6.miRNA.interval Transcript coordinates], [http://homes.gersteinlab.org/people/lh372/VAT/gencode6.miRNA.fa Transcript sequences]
 +
** GENCODE version 7 (hg19): [http://homes.gersteinlab.org/people/lh372/VAT/gencode7.miRNA.interval Transcript coordinates], [http://homes.gersteinlab.org/people/lh372/VAT/gencode7.miRNA.fa Transcript sequences]

Latest revision as of 15:33, 15 June 2011

VAT Main Page

Contents


External Software

Top

Required

  • GSL - GNU Scientific Library (version-1.14; required for libBIOS, which is a general C library).
  • BlatSuite - BLAT and a collection of utility programs. These tools are utilized by VAT. Note: these executables must be part of the PATH.
  • GD library - The GD library is used to create an image for each gene model and its associated variants (version-2.0.35; required by VAT).
  • Tabix - Tabix (version-0.2.3) is a generic tool that indexes position-sorted files in tab-delimited formats to facilitate fast retrieval (download). These tools are utilized by VAT. Note: these executables must be part of the PATH.
  • RNAstructure - RNAstructure is a software package for RNA structure prediction and analysis. This tool is utilized by VAT for prediction of structures for RNA sequences with and without the variants.
  • VARNA - VARNA is a java applet for producing high quality RNA secondary structure plots. VAT utilizes VARNA for visualization of the RNA secondary structures.


Top

Optional

  • VCF tools - VCF tools consists of a suite of useful modules to manipulate VCF files.



VAT Download


Important Note
==============

THIS PACKAGE (VAT) IS PROVIDED "AS IS" AND WITHOUT ANY EXPRESSED OR IMPLIED
WARRANTIES, INCLUDING, WITHOUT LIMITATION, THE IMPLIED WARRANTIES
OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE.


Top

Source code

VAT is a based on a general C library, called libBIOS. A TAR ball of libBIOS and VAT can be downloaded here:


Top

Executables

Statically built binaries for UNIX can be found here:


Top

License information

The software package is released under the Creative Commons license (Attribution-NonCommerical).
For more details please refer to the Permissions Page on the Gerstein Lab webpage.



Installation

Top

Installation of the external GSL and GD libraries

In order to install VAT two external libraries must be installed first. The libBIOS library depends on GSL, whereas VAT makes use of the GD library. Please follow the instructions provided by each package. The GSL library can be installed on most systems using the following commands (for details, please refer to the specific instructions at the GNU Scientific Library website):

$ cd /path/to/gsl-1.14/
$ ./configure --prefix=`pwd`
$ make
$ make install

Similarly, the GD library can be installed on most systems with the following commands:

$ cd /path/to/gd-2.0.35/
$ ./configure --prefix=`pwd` --with-jpeg=/path/to/jpegLib/
$ make
$ make install

After they are installed, the first step to install VAT is the installation and configuration of libBIOS.


Top

Installation and Configuration of libBIOS

Depending on where the three libraries (GSL, libBIOS, and GD) are installed, the following variables need to be set:

export CPPFLAGS="-I/path/to/gsl-1.14/include -I/path/to/libbios/include -I/path/to/gd-2.0.35/include"
export LDFLAGS="-L/path/to/gsl-1.14/lib -L/path/to/libbios/lib -L/path/to/gd-2.0.35/lib"

libBIOS can be installed on most systems with the following commands:

$ cd /path/to/libbios-x.x.x/
$ ./configure --prefix=`pwd` 
$ make
$ make install


Top

Installation of RNAstructure and VARNA

Download RNAstructure and follow the building instructions. Make sure that the build directory is included in PATH environment variable. In addition, RNAstructure needs an environment variable named DATAPATH to be set to the directory of thermodynamic parameter files that are distributed with RNAstructure package.

Download VARNA jar file and add the jar file path to CLASSPATH environment variable.


Top

Installation and Configuration of VAT

A few simple steps are required to install VAT:

$ cd /path/to/vat-x.x.x/
$ ./configure --prefix=`pwd` 
$ make
$ make install

VAT contains a configuration file (vatConfirgurationTemplate.txt), which contains a set of variables that are used by a number of different programs. The name/value pairs are space or tab-delimited. Empty lines are lines starting with '//' are ignored.


// ===============================================================================
// REQUIRED
// ===============================================================================

// Tabix directory (includes both tabix and bgzip)
TABIX_DIR /path/to/tabix-0.2.3


// ===============================================================================
// OPTIONAL (required only for CGIs)
// ===============================================================================

// CGI base URL (where the CGIs are located)
WEB_URL_CGI http://webserver.org/path

// Path to the web data directory where the preprocessed files are stored
WEB_DATA_DIR /path/to/public_html/path/to/VAT
// URL to preprocessed files
WEB_DATA_URL http://webserver.org/path/to/VAT

This file has to be configured properly by filling in the required information. Subsequently, the following environment variable (VAT_CONFIG_FILE) has to be set:

VAT_CONFIG_FILE=/pathTo/vat/vatConfirgurationTemplate.txt



Setup of the web server


This step is optional, but useful for visualizing the results of processed data sets. The following steps are required:

  • The executable vat_cgi has to be located in the cgi-bin directory on the web server
  • The configuration file (vatConfirgurationTemplate.txt) must contain the pertinent information
  • The following .htaccess file should be added to the cgi-bin:
SetEnv VAT_CONFIG_FILE /path/to/vatConfirgurationTemplate.txt
  • The web data directory (defined by WEB_DATA_DIR in the configuration file) requires the following information:

For additional information please refer to the example workflow.



Download of pre-processed annotation sets


The following annotation sets are derived from the GENCODE project. Each each entry has a set of transcript coordinates (in Interval format) and a set of transcript sequences (introns removed; sequence with respect to the '+' strand; in FASTA format)


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